U251-Luc
CBP30207L
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| I. Introduction | |
| Host Cell: | U251 |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+1%Non Essential Amino Acids NEAA+1mM Sodium Pyruvate NaP+10%FBS+2ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens,human |
| Tissue: | Brain |
| Disease: | Human glioblastoma astrocytoma |
| Morphology: | Pleomorphic/astrocytoid |
| Growth Properties: | Adherent |
| III. Representative Data | |
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Figure. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). The number of Luminescence signal is 508±6(Mean±SD) on U251 cells while 49992742 ±1810197(Mean±SD) on U251-Luc cells. |
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| IV. Stablity Test | |
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Figure. Stability test results. U251-Luc cells showed at least 32 passage stability with similar luminescence unit. |
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