Hep3B-Luc
CBP30219L
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| I. Introduction | |
| Host Cell: | Hep3B |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM+10%FBS+1%Non Essential Amino Acids NEAA+1mM Sodium Pyruvate NaP+2μg/ml Puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Liver |
| Disease: | NA |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
 Figure 1. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly recommend purchasing from Cobioer). The luminescence signal is 620 ± 24 (Mean ± SD) on Hep3B cells while 2,882,860 ± 187,087 (Mean ± SD) on Hep3B-Luc cells, and Hep3B-Luc showed at least 32 passage stability with similar luminescence unit. |
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| IV. In VIVO Data | |
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Index |
Content |
|
Animal |
BALB/c-nude |
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Implanted Position |
肝原位 |
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Injected Cell Numbers |
2×10^6/20μL+matrigel |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. Hep3B-Luc Orthotopic Tumor Model.
2. Tumor Flux
Figure 3. Hep3B-Luc Orthotopic Tumor Model.
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
