hGPC1/4T1
CBPG0252
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| I. Background | |||
| GPC1 基因（Glypican-1）位于人类染色体 2q37.3 区域，编码一种含 558 个氨基酸的细 胞表面硫酸乙酰肝素蛋白聚糖。该蛋白顺利获得硫酸肝素链与细胞外基质及生长因子（如 Hedgehog、Wnt）结合，调控细胞信号传导、增殖及分化过程。GPC1 在多种肿瘤中高表达， 包括胰腺癌、乳腺癌和非小细胞肺癌，顺利获得促进血管生成和上皮-间质转化（EMT）参与肿 瘤进展，其血液表达水平在胰腺癌早期诊断中显示出高敏感性和特异性，成为潜在生物标 志物。此外，GPC1 还与神经系统疾病（如脊髓性肌萎缩症）相关，并参与朊病毒蛋白转 化。研究聚焦于其作为肿瘤治疗靶点的价值，包括靶向纳米药物开发及信号通路调控机制 探索，为癌症精准治疗给予新方向。 | |||
| II. Introduction | |||
| Host Cell: | 4T1 | ||
| Expressed gene: | GPC1 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | RPMI-1640+10%FBS+ 2μg/ml Puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mouse | ||
| Tissue: | Mammary gland | ||
| Morphology: | Epithelial | ||
| Growth Properties: | Adherent | ||
IV. Representative Data Figure 1.Recombinant hGPC1/4T1 stably expressing GPC1. |
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